Genetic Diversity Of Locally Rice Germplasm From Tana Toraja and Enrekang Based On RAPD (Random Amplified Polymorphism DNA) Markers

Abstract

This study aims to determine the genetic diversity of locally rice germplasm based on RAPD analysis. Totally eighten of locally rices were used in this research : Pare Ambo, Pare Bau, Pare Birrang, Pare Bumbungan, Pare Kobo, Pare Lalodo, Pare Lea, Pare Rogon, Pare Tallang, Pare Kamida, Pare Lambau, Pare Solo, Pulu Mandoti, Pare Lotong, Pallan, Mansur, Pare Pinjan and Pare Salle. Extraction of DNA was made according the method of Doley and Doley (1987) with modification. Molecular analysis was performed using six different RAPD primers, there were OPF-14, OPH-14, OPM-05, OPM-12, OPU-15 and OPX-09. PCR products were separated on 1.8% agarose gel and vizualised in EtBr solution. DNA bands were then analyzed with NTSYS program. The results showed a highly variation in the DNA band profiles, the bands number of each primer varied between 4-10 bands with an average of 7 bands from a total of 42 bands. Primers that generated most polymorphic DNA fragments are OPF-14 and OPU-15, as many as 10 fragments/bands. Dendrogram analysis separated 18 local rice accessions in three groups. Pare Pallan, Mansur, Lambau, Solo, Tallang and Lotong has similarity cooeficient of 96 %, while Pare Bumbungan has farther most similarity with other rice genotypes that only 35 %.