Abstract:
The research on extraction bioactive compound of cocoa pod husk waste and
antifungal activity assay by in vitro has been done. The aim of research was to get a
phytochemical components extracted from the rind of cocoa, phenolic content and test the
bioactivity of extract cocoa pod husk against on pathogenic fungus Fusarium oxysporum. Pod
husks used are dried. Samples were extracted by maceration with acetone-water (7: 3) and 70%
ethanol. Each extracts were then analyzed using Gas Chromatography-Mass Spectrometry
(GC-MS) to determine the active compounds found in the extract cocoa pod husk. The results
obtained by GC-MS analysis showed three major components of the compound in ethanol ie:
Methyl-9,12-Dienoate Octadeca; 9-octadecenoic acid (Z) -, Methyl Ester; Hexadecanoic acid,
15-methyl, Methyl Ester. Whereas in acetone solvent compounds showed 4 major components,
namely: Isopropyl myristate; 1,2-Benzenedicarboxylic Acid, Dioctyl Ester; 9-octadecenoic
acid (Z) -, Methyl Ester; Octadecanoic Acid, Methyl Ester. Acetone extract phenolic levels of
94.92 mg GAE / g extract and ethanol extract of 49.92 GAE / g extract. Antifungal testing by
the agar diffusion method showed of extract cocoa pod husk with acetone able to inhibit the
growth of the fungus Fusarium oxysporum (fungus pathogen on tomatoes), meaning that the
extract has the potential to be developed as natural fungicides to suppress the growth of
pathogenic fungi.
