Abstract:
The sperm sexing technique is the separation of spermatozoa carrying the X
chromosome and the Y chromosome, which allows farmers to choose male births for beef
cattle businesses. However, the process of centrifugation and sperm preservation can cause
oxidative stress which gives rise to free radicals. Therefore, an antioxidant ingredient is
needed in the diluent, including noni fruit extract which contains lots of flavonoids. This
research aims to analyze the addition of noni fruit extract in a diluent to improve the quality
of Y-chromosome spermatozoa at a storage temperature of 5oC. The results of the study
showed the results of analysis of the progressive motility of Y-chromosome spermatozoa,
showing a decrease in progressive motility, on D7 and D-9 there was a difference between
control vs P1 and P2 (P<.05) respectively 37.63% vs 49.96% vs 48.56%, while on day 11
showed a very real difference between P0 vs P1 vs P2 respectively 24.32% vs 36.77% vs
40.87%. The addition of 3% and 5% noni extract can improve the quality of Y spermatozoa
stored at 5oC. This is because noni extract contains amino acids, ascorbic acid, and
flavonoids as antioxidants so that it is able to prevent free radicals, scavengers, and damage
to the spermatozoa plasma membrane can be prevented. The addition of 5% noni fruit
extract in the diluent to improve the quality of Y-chromosome spermatozoa at a storage
temperature of 5oC for up to 11 days
